Technical Data
S8400-08
Surfactant Protein A (SP-A)
Description:
Surfactant protein A (SP-A) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-D and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45ng/ml in healthy individuals.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653. Female CF1 x Balb/c mice immunized.

Culture Medium:
RPMI 1640 with 10% fetal calf serum

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6F187Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Native SP-A purified from bronchioaveolar lavage from proteinosis patients adsorbed onto aluminum hydroxide gel.
Purity:
Purified by Protein A/G affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes human pulmonary SP-A. In Western Blot after SDS-PAGE it reacts with reduced forms of SP-A and weaker with non-reduced forms. Reacts specifically with human SP-A in immunohistochemical staining from paraffin embedded tissue samples. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. Reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Holmskov, U.L., APMIS Suppl. 100: 1-59 (2000). 2. Johansson, J. & Curstedt, T., Eur. J. Biochem. 244: 675-693 (1997). 3. Madsen, J., et al., Am. J. Respir. Cell Mol. Biol. 29: 591-597 (2003).