		Technical Data
S8400-08	Surfactant Protein A (SP-A)

Description:
Surfactant protein A (SP-A) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular “head” regions linked by triple-helical, collagen-like, strands. This group also includes SP-D and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45ng/ml in healthy individuals.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.

Culture Medium:
RPMI 1640 with 10% fetal calf serum

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	6F187	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Native SP-A purified from bronchioaveolar lavage from proteinosis patients adsorbed onto aluminum hydroxide gel.

Purity:

Purified by Protein A/G affinity chromatography

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Specificity:

Recognizes human pulmonary SP-A. In Western Blot after SDS-PAGE it reacts with reduced forms of SP-A and weaker with non-reduced forms. Reacts specifically with human SP-A in immunohistochemical staining from paraffin embedded tissue samples. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. Reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Holmskov, U.L., APMIS Suppl. 100: 1-59 (2000). 2. Johansson, J. & Curstedt, T., Eur. J. Biochem. 244: 675-693 (1997). 3. Madsen, J., et al., Am. J. Respir. Cell Mol. Biol. 29: 591-597 (2003).