Technical Data
S8500-03G
Survivin
Description:
Regulated inhibition of programmed cell death (apoptosis) preserves normal homeostasis and tissue and organ morphogenesis. Aberrations in this process contribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have been found in various species, including human. Inhibitor of Apoptosis (IAP) proteins contain one/three Cys/His baculovirus IAP repeats plus a C-terminus RING finger and are thought to block an evolutionary conserved step in apoptosis. Survivin encodes a structurally unique IAP. Present during fetal development, survivin is undetectable in terminally differentiated adult tissues. However, survivin becomes prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. Survivin is also found in high-grade non-Hodgkin’s lymphomas. These data suggest that apoptosis inhibition may be a general feature of neoplasia and identify survivin as a potential new target for apoptosis-based therapy in cancer and lymphoma.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa whole cell extract

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k3H1Affinity Purified
SizeStorageShippingSourceHost
50ul-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant human survivin, full-length. Epitope: aa57-67. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid, PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the cytoplasmic form of human survivin (~16.5kD). Species Crossreactivity: rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fortugno, P., et al., J. of Cell Science. 115(3): 575-585 (2002). 2. Zhu, L., et al., FEBS Letters. 508:369-374 (2001). 3. Xu, Z., et al., J. Biological Chemistry. 279: 1838-1844 (2004). 4. Ambrosini, G., et al., JBC. 273(18): 11,177-11,182 (1998). 5. Li, F., et al., Nature Cell Biology. 1(8): 461-466 (1999). 6. Grossman, D., et al., J. of Invest Dermatology, 113(6): 1076-1081 (1999). 7. Li, F., et al., Nature. 396: 580-584 (1998). 8. Grossman, D., et al., J. Invest. Dermatology. 113: 1076-1081 (1999). 9. Adida, C., et al., Blood. 96(5): 1921-1925 (2000).