Technical Data
Syk, a protein tyrosine kinase (PTK) is expressed in immature T cells, all B cells, basophils, hepatocytes, platelets and erythrocytes. It functions in some T cell receptor signalling processes in addition to B cell receptor and integrin-mediated signal transduction processes. Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors. It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis. There is also evidence of a role for Syk in nonimmune cells, and Syk is a potential tumor suppressor in human breast carcinomas.

Suitable for use in Immunocytochemistry, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 5ug/ml
Western Blot: 1-2ug/ml, 60 minutes
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG16D784Affinity Purified
100ug-20CBlue IceHumanMouse
Recombinant fragment corresponding to aa5-360 of human Syk.
Purified by Protein A affinity chromatography
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide
Recognizes human Syk. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Amoui, M., et al., Eur. J. Immunol. 27: 321 (1997). 2. Tolar, P., et al., Eur. J. Immunol. 27: 3389 (1997). 3. Swann, P.G., et al., J. Biol. Chem. 274: 23,068 (1999). 4. Arudchandran, R., et al., J. Exp. Med. 191: 47 (2000). 5. Manetz, T.S., et al., Mol. Cell. Biol. 21: 3763 (2001). 6. Kovarova, M., et al., Mol. Cell. Biol. 21: 8318 (2001). 7. Halova, I., et al., Int. Immunol. 14: 213 (2002). 8. Parravicini, V., et al., Nat. Immunol. 3: 741 (2002).