Technical Data
S9108-03A
Synaptophysin, CT
Description:
Synaptophysin (SypI), also referred as p38 (1), constitutes approximately 7% of the total vesicle protein and is selectively associated with small synaptic vesicles (SSVs)
Syp I contains four transmembrane regions and can be phosphorylated on its cytoplasmic C-terminus by both serine/threonine and tyrosine kinases (2). In addition to syp I, synaptic vesicles contain a highly homologous membrane protein named synaptoporin or synaptophysin II (Syp II) (3). While Syp I is ubiquitously expressed and present in virtually all synapses whereas Syp II is expressed at high levels only in selected neurons (4).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:10,000 (34kD)
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k388Supernatant
SizeStorageShippingSourceHost
100ul 4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Synaptophysin. MW: 34kD
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH7.4, 0.15M NaCl, 0.01% sodium azide, 0.05% BA and 40% glycerol.
Specificity:
Recognizes human Synaptophysin. Crossreactivity: Mouse and rat. Species sequence homology: Bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Navone F, Jahn R, Di Gioia G, Stukenbrok H, Greengard P, De Camilli P. J Cell Biol. Dec;103(6 Pt 1):2511-27 (1986 ). 2. Linstedt, A. D., Vetter, M. L., Bishop, J. M. & Kelly, R. B. J. Cell Biol. 117: 1077-1084.(1992) 3. Knaus, P., Marqueze-Pouey, B., Scherer, H. & Betz, H. Neuron 5: 453-462.(1990) 4. Marqueze-Pouey, B., Wisden, W., Malosio, M. L. & Betz, H. J. Neurosci. 11: 3388-3397. (1991)