Technical Data
S9108-03A
Synaptophysin, CT
Description:
Synaptophysin (SypI), also referred as p38 (1), constitutes approximately 7% of the total vesicle protein and is selectively associated with small synaptic vesicles (SSVs)
Syp I contains four transmembrane regions and can be phosphorylated on its cytoplasmic C-terminus by both serine/threonine and tyrosine kinases (2). In addition to syp I, synaptic vesicles contain a highly homologous membrane protein named synaptoporin or synaptophysin II (Syp II) (3). While Syp I is ubiquitously expressed and present in virtually all synapses whereas Syp II is expressed at high levels only in selected neurons (4).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:10,000 (34kD)
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG6k388Supernatant
SizeStorageShippingSourceHost
100ul 4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Synaptophysin. MW: 34kD
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH7.4, 0.15M NaCl, 0.01% sodium azide, 0.05% BA and 40% glycerol.
Specificity:
Recognizes human Synaptophysin. Crossreactivity: Mouse and rat. Species sequence homology: Bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Navone F, Jahn R, Di Gioia G, Stukenbrok H, Greengard P, De Camilli P. J Cell Biol. Dec;103(6 Pt 1):2511-27 (1986 ). 2. Linstedt, A. D., Vetter, M. L., Bishop, J. M. & Kelly, R. B. J. Cell Biol. 117: 1077-1084.(1992) 3. Knaus, P., Marqueze-Pouey, B., Scherer, H. & Betz, H. Neuron 5: 453-462.(1990) 4. Marqueze-Pouey, B., Wisden, W., Malosio, M. L. & Betz, H. J. Neurosci. 11: 3388-3397. (1991)