Technical Data
Synaptophysin (Major Synaptic Vesicle Protein p38, SYP)
Synaptophysin (p38) is an abundant integral membrane protein of small synaptic vesicles in the brain. It is also present in endocrine cells, where it is associated with small electron-translucent vesicles. Synaptophysin expression has been widely used as a marker for synaptogenesis in developmental studies in vivo and in vitro and as a marker for neuron-specific cell lineages in tumors of the central nervous system and of peripheral neuroendocrine cell derivation. It has multiple transmembrane regions and a cytoplasmic tail that contains 10 copies of a tyrosine-rich repeat. Biochemical experiments have demonstrated that it transverses the membrane four times and has cytoplasmic carboxyl- and amino-termini. It also contain unstable intramolecular disulfide bonds that spontaneously rearrange, thereby creating higher-order polymers that are larger than the native protein and that have distinctly different biochemical properties. Mutations involving the synaptophysin gene may be responsible for an X-linked disorder. Chromosomal localization of the human gene for synaptophysin established the human SYP locus on the X chromosome in subbands Xpll.22-pll.23. It is involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceHumanMouse
Not determined.
Synthetic peptide corresponding to a region near the c-terminal end of the synaptophysin protein. Cellular Localization: Cytoplasmic.
Supplied as a liquid, 0.05% sodium azide.
Recognizes human Synaptophysin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blumenfiel et al. Arch Pathol Lab Medicine 120: 478, 1996. 2. Dottorini et al. Cancer 77: 1556, 1996. 3. Oezcelik et al. Am J Hum Genet 47: 551, 1990.