Technical Data
S9108-11
Synaptophysin
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labelled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.

Applications:
Suitable for use in Immunohistochemistry, Immunoflourescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry/Immunoflourescence (Frozen, paraffin): 1:100-1:200 using indirect immunoperoxidase on formalin-fixed, paraffin embedded rat cerebellum.
Western Blot: 1:500 detected Synatophysin on 10ug of mouse brain lysates.
Optimal dilution determined by the researcher.

Control:
Brain Tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q105Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceRatMouse
Concentration:
Not Determined
Immunogen:
Rat retina synaptophysin (Accession# NM_003179.2)
Purity:
Ascites
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes rat Synaptophysin at ~40kD. Localizes the 38kD band of rat synaptophysin by immunoblotting. Stains synaptophysin in neurons, neuromuscular junctions, paraganglia cells, pancreatic islet cells, anterior lymphomas and adrenal cells. Detects synaptophysin in many types of benign and malignant neural and epithelial neuroendocrine neoplasmas. Species crossreactivity: human, guinea pig, porcine, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anna. N.Y. Acad. Sci. (1987) 493:493. Nosrat, I.V., et al., Developmental Biology (2001). 2. Nosrat, I.V., et al., Developmental Biology (2001) on line. 3. Harper, J. M. et al. (2004) Proc. Nat. Acad. Sci. (USA) 101(18): 7123-7128. 3. Magvai, S., et al., J. Neuroscience (2005) 25:10729-10729. 4. Calon, F. et al. (2005) Eur J Neurosci. 22: 617-626.