		Technical Data
S9108-25B	Synaptophysin 2 (DKFZp686G0883, MGC26651, Synaptoporin, SPO, SYNPR)

Description:
Synaptophysin 2, also known as Synaptoporin, is a component of the synaptic vesicle membrane and thought to play an important role in synaptic vesicle trafficking (Dai et al., 2003). It is highly homologous to Synaptophysin 1, yet differs in expression. Whereas Synaptophysin 1 is ubiquitiously expressed, Synaptophysin 2 is concentrated in the mossy fiber synapses of the hippocampus (Grabs et al., 1994; Singec et al., 2002).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat hippocampal lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Rat	Rabbit

Concentration:

Not determined

Immunogen:

Peptide corresponding to amino acid residues from the C-terminal region of rat Synaptophysin 2 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug per ml BSA, 50% glycerol.

Specificity:

Recognizes rat Synaptophysin 2. Species sequence homology: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Dai J, Ji C, Gu S, Wu Q, Wang L, Xu J, Zeng L, Ye X, Yin G, Xie Y, Mao Y (2003) Cloning and sequence analysis of the human cDNA encoding the synaptoporin, a highly conserved synaptic vesicle protein. Molecular Biology Reports 30:185-191. 2. Singec I, Knoth R, Ditter M, Hagemeyer CE, Rosenbrock H, Frotscher M, Volk B (2002) Synaptic vesicle protein synaptoporin is differently expressed by subpopulations of mouse hippocampal neurons. J Comp Neurol 452(2):139-153. 3. Grabs D, Bergmann M, Schuster T, Fox PA, Brich M, Gratz M (1994) Differential expression of synaptophysin and synaptoporin during pre- and postnatal development of the rat hippocampal network. Eur J Neurosci. 6(11): 1765-71.