Technical Data
S9109-19D
Synaptotagmin 1, Phosphorylated (Ser309) (Synaptotagmin-1, SYT1, Synaptotagmin I, SytI, P65) (Azide Free)
Description:
Synaptotagmin 1 is a 60kD member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding.

Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Phosphopeptide corresponding to aa sequence surrounding phosphorylated serine 309 of synaptotagmin 1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM Hepes, pH 7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.
Specificity:
Recognizes rat synaptotagmin 1 when phosphorylated at Ser309. Species sequence homology: Bovine, Chicken, Human, Zebrafish, Mouse, Dog, Primate.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Xu, Jun. et al. (2007) Synaptotagmin-1, -2, and -9: Ca2+ Sensors for Fast Release that Specify Distinct Presynaptic Properties in Subsets of Neurons. Neuron. Volume 54:567-581. 2. Sullivan, J. (2007) Please Release Me. Neuron. Volume 54:493-494. 3. Hilfiker, S. et al. (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases. J. Neurochem. 73:921-932.