		Technical Data
S9200-02A	Syntaxin (Synaptotagmin-Associated 35kD Protein, Neuron-Specific Antigen HPC-1)

Description:
Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain (Habc), a SNARE domain (known as H3), and a single C-terminal transmembrane domain. The SNARE(H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex.

Applications:
Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:5000
Western Blot: 1:1000
Optimal working dilutions to be determined by researcher.

Recommended Secondary Antibodies:
F(ab')2 rabbit anti-mouse IgG HRP

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSO myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	3H2896	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human synaptic proteins.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes human syntaxin. Species Crossreactivity: Porcine, hamster, rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Honer, W.G. et al. (1993). Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609: 9-20.