Technical Data
S9200-02A
Syntaxin (Synaptotagmin-Associated 35kD Protein, Neuron-Specific Antigen HPC-1)
Description:
Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain (Habc), a SNARE domain (known as H3), and a single C-terminal transmembrane domain. The SNARE(H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex.


Applications:
Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:5000
Western Blot: 1:1000
Optimal working dilutions to be determined by researcher.

Recommended Secondary Antibodies:
F(ab')2 rabbit anti-mouse IgG HRP

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSO myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H2896Affinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human synaptic proteins.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human syntaxin. Species Crossreactivity: Porcine, hamster, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Honer, W.G. et al. (1993). Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609: 9-20.