Technical Data
S9205-01
Syntaxin (Synaptotagmin-Associated 35kD Protein, Neuron-Specific Antigen HPC-1)
Description:
Syntaxin is a 35kD type II integral membrane protein localized to the plasma membrane at nerve terminals (2). Syntaxin was identified through its association with synaptotagmin and N-type calcium channel, a subtype of calcium channel on the plasma membrane important for neurotransmitter release. Homologs of syntaxin were identified in various eukaryotes ranging from yeast to human (1, 2). Evidences for syntaxinís involvement in vesicular transport and neurotransmission include the requirement of its yeast homologs, SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane (3). Syntaxin is also the target of clostridial neurotoxin C1 that block neurotransmitter release (4). The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF (5).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot (Colorimetric) (1,6): 1.0ug/ml
Immunohistochemistry: 1:2000
Immunoprecipitation: 1:500
Optimal dilutions to be determined by the researcher.
PC-Personal Communication

Positive Controls:
Rat Brain Tissue Extract

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG14E209Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Mice were immunized with synaptic vesicle-containing fractions that were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS 0.1mM PMSF, 50% glycerol.
Specificity:
Recognizes human syntaxin at~38kD. No reactivity was seen with a 35kDa protein in mouse liver extracts. Some reactivity with higher molecular weight bands of unknown identity is observed on immunoblots. Species Crossreactivity: mouse, rat, porcine, feline, bovine, hamster, Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Honer, W.G., Hu, L. and Davies, P.; Brain Research 609: 9-20 (1993). 2. Calakos, N. and Scheller, R.; Physiological Reviews 76: 1-29 (1996). 3. Aalto, M.K., Ronne, H. and Keranen, S.; EMBO J. 12: 4095-4104 (1993). 4. Blasi, J., Chapman, E.R., Yamasaki, S., Binz, T., Niemann, H. and Jahn, R.; EMBO
J. 12: 4821-4828 (1993). 5. Sollner, T., Bennett, M.K., Whiteheart, S.W., Scheller, R.H. and Rothman, J.E.; Cell 75: 409-418 (1993). 6. Honer, W.G., et. al.; Neuroscience 1: 99-110 (1997).