Technical Data
Synuclein, alpha (SNCA, 127750, P37840, MGC110988, NACP, PARK1, PARK4, non-A beta Component of AD Amyloid, NACP, Parkinson Disease Familial 1, PD1)
alpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer's Disease (AD). Alpha-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including alpha-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 shows a band at 14-19kD
Immunohistochemistry (frozen or paraffin sections):
Frozen sections: 1:4000-1:12,000
Paraffin sections: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human brain sections

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, ddh2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Not Determined
Human alpha-synuclein aa111-131 (KLH coupled).
Supplied as a lyophilized powder. No preservative added. Reconstitute with 50ul sterile 40-50% glycerol, ddH2O.
Recognizes human synuclein, alpha. Immunohistochemistry and Western Blot analysis of human brain indicates a high level of specificity. Crossreactivity with beta-synuclein is minimal. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Iwai, A, et al., Neuron (1995) 14:467475. 2. Spillantini, MG, Nature (1997) 388:839840. 3. Gai, WP, et al., Lancet (1998) 352(9127):547548. 4. Abeliovich, A, et al., Neuron (2000) 25:239252.