Technical Data
Synuclein, alpha
Human a-Synuclein is predominantly a presynaptic neuronal protein that has been localized in close proximity to synaptic vesicles. Studies have revealed that this protein is mostly cytosolic, although a fraction may be associated with vesicular membranes. In addition, a-synuclein binds to small synthetic unilamellar vesicles and rat brain vesicles, suggesting that a-synuclein may play a role in neurotransmission or in the organization and regulation of synaptic vesicles. a-Synuclein has been reported to be a major component of Lewy bodies in Parkinson’s disease, as well as dementia and Alzheimer’s disease. In recent studies, antibodies to b- and g-synuclein have been used to investigate the biological functions of a-synuclein as well as its role in neurodegenerative disorders.

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry: paraffin tissues
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1,k3G281Affinity Purified
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Recombinant human alpha-synuclein
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Recognizes human alpha-synuclein protein. Does not crossreact with beta or gamma synuclein or synoretin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Giasson B., et. al. A panel of epitope-specii c antibodies detects protein domains distributed throughout human a-Synuclein in Lewy Bodies of Parkinson’s Disease. J Neurosci Res. 59:528–533 (2000). 2. Baba M., et. al. Aggregation of alpha-synuclein in Lewy bodies of sporadic Parkinson’s disease and dementia with Lewy bodies. Am J Pathol. 152(4): 879–884 (1998).