Technical Data
S9500-26
Synuclein, alpha
Description:
Human a-Synuclein is predominantly a presynaptic neuronal protein that has been localized in close proximity to synaptic vesicles. Studies have revealed that this protein is mostly cytosolic, although a fraction may be associated with vesicular membranes. In addition, a-synuclein binds to small synthetic unilamellar vesicles and rat brain vesicles, suggesting that a-synuclein may play a role in neurotransmission or in the organization and regulation of synaptic vesicles. a-Synuclein has been reported to be a major component of Lewy bodies in Parkinson’s disease, as well as dementia and Alzheimer’s disease. In recent studies, antibodies to b- and g-synuclein have been used to investigate the biological functions of a-synuclein as well as its role in neurodegenerative disorders.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml
Immunohistochemistry: Paraffin sections
Immunoprecipitation: Native
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3G281Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to human alpha-synuclein.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Specificity:
Recognizes human alpha-synuclein protein. Does not crossreact with beta or gamma synuclein or synoretin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Giasson B., et. al. A panel of epitope-specii c antibodies detects protein domains distributed throughout human a-Synuclein in Lewy Bodies of Parkinson’s Disease. J Neurosci Res. 59:528–533 (2000).