		Technical Data
T0709-65	TAG (TIRAP3b)

Description:
TAG:TIRAP3:TICAM2 is a member of the Toll:Interleukin 1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). It undergoes post-translational modifications such as myristoylation and phosphorylation. TAG is a homodimer interacting with TLR4, TICAM1, IRF3 and IRF7 in response to LPS activation. The LPS-TLR4 signaling cascade regulates the MYD88-independent pathway during innate immune response. It is also involved in IL-1 triggered NF-kappa-B activation. It physically binds TLR4 and TICAM1 to transmit LPS-TLR4 signal to TICAM. Expression of TIRAP3 appears to be essential for TLR4 signaling.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa150-200 of TAG/TIRAP3b

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes TAG/TIRAP3b. Species Crossreactivity: chimpanzee, bovine, mouse, opossum, rat, rhesus monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Selvarajoo, S. et al. PLoS One. 3:e3430 (2008). 2. Sullivan, C. et al. J. Immunol. 178:4517-4527 (2007). 3. Tanimua, N. et al. Biochem. Biophys. Res. Commun. 368:94-99 (2008).