Technical Data
TAG:TIRAP3:TICAM2 is a member of the Toll:Interleukin 1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). It undergoes post-translational modifications such as myristoylation and phosphorylation. TAG is a homodimer interacting with TLR4, TICAM1, IRF3 and IRF7 in response to LPS activation. The LPS-TLR4 signaling cascade regulates the MYD88-independent pathway during innate immune response. It is also involved in IL-1 triggered NF-kappa-B activation. It physically binds TLR4 and TICAM1 to transmit LPS-TLR4 signal to TICAM. Expression of TIRAP3 appears to be essential for TLR4 signaling.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceRabbit
Synthetic peptide corresponding to aa150-200 of TAG/TIRAP3b
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes TAG/TIRAP3b. Species Crossreactivity: chimpanzee, bovine, mouse, opossum, rat, rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Selvarajoo, S. et al. PLoS One. 3:e3430 (2008). 2. Sullivan, C. et al. J. Immunol. 178:4517-4527 (2007). 3. Tanimua, N. et al. Biochem. Biophys. Res. Commun. 368:94-99 (2008).