|Molecular Biology||Storage: -20°CShipping: Blue Ice|
5'-A C G T^-3'
3'-^T G C A-5'
Tail-neoschizomer of MaeII, produces DNA fragments that have a 4-base 3’-extension
Thermus aquaticus Cc1–331
Restriction Enzyme Buffer E, 10X; R1625-04, Included: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 65°C**
Dilute with 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with TaiI.
After 50-fold overdigestion (3 units/ug DNA x 17 hours) with TaiI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.5uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay:
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
TaiI does not cut Am5CGT. Blocked by CG methylation.
Stability during Prolonged Incubation:
A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 65°C.
Enzyme is not inactivated by incubation at 80°C for 20min.
Number of Recognition Sites in DNA:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.