Technical Data
T0850
Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD)
Description:
Tamm-Horsfall glycoprotein (THP), which is synthesized by renal tubular cells, is the most abundant protein in normal human urine. Although its physiological function remains unclear, it has been proposed that THP may act as a defense factor against urinary tract infections by inhibiting the binding of S- and P-fimbriated Escherichia coli to renal epithelial cells. Because THP-related proteins are also found in the superficial layers of the oral mucosa, THP may interfere with the cytoadherence of pathogenic bacteria that colonize mucosal surfaces other than those of the urogenital tract. It has been shown that THP binds to virulent Bordetella pertussis and reduces its adherence to both renal and pulmonary epithelial cells. This cytoadherence inhibitory effect was not observed with a B. pertussis mutant lacking the pertussis toxin (PTX) operon, and was dependent on the direct interaction of THP with the S2 subunit within the PTX B oligomer. It has also been shown that the glycosylation moiety of THP is crucial for its binding to PTX. The THP-PTX interaction was exploited to develop an affinity chromatography method that allows a one-step purification of active PTX. These observations suggest that besides its anti-adherence activity, THP may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces.

Applications:
Suitable for use in IEP, RID, Double Diffusion and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
IEP: 100ul antiserum vs. 5ul uromucoid (50mg/L)
RID and Rocket RID: 10ul antiserum/cm2 in gel vs. 5ul uromucoid (50mg/L), NT - 1/5
Double Diffusion: 10ul antiserum vs. 3ul uromucoid (50mg/L)
Note: The use of 3% PEG 6000, 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended for the above applications.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanSheep
Concentration:
Not Determined
Immunogen:
Highly purified human Tamm-Horsfall glycoprotein (uromucoid) from urine (SDS-PAGE > 95%).
Purity:
Serum
Form
Supplied as a liquid in GBS, pH 7.4, 0.1% sodium azide, 0.1% EACA, 0.01% benzamidine, 1mM EDTA.
Specificity:
Recognizes human Tamm-Horsfall Glycoprotein. One arc by 2D-IEP against Tamm-Horsfall glycoprotein (1mg/ml). No precipitin arc by IEP against normal human plasma. Identity verified by double immunodiffusion.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Smith, L.A. et al., (2006) J Am Soc Nephrol 17: 2821-2831. 1. Tamm & Horsfall, F.L., Proc. Soc. Exp. Biol. Med. 74: 108-114 (1950). 2. Kidney Int. 16: 279-289 (1979). 3. Science 236: 83-87 (1987). 4. Science 237: 1479-1484 (1987). 5. Menozzi, F.D., et al., Microbiology. 148(4): 1193-1201 (2002).