Technical Data
T1030
Tau (Tau Protein, Microtubule-associated Protein, MAP)
Description:
Tau is a heterogeneous group of proteins that range in molecular weight from 50-67kD. They are instumental in the assembly of microtubules and have been associated with some disease states. In normal brain, tau is localized in the axons of the neurons but in some neuropathological lesions, it accumulates within the body of the neuron. Tau protein is a microtubule-associated protein predominantly localized to neuronal axons. Tau promotes tubulin polymerization and the stabilization of microtubules. In vivo this protein is enriched in the exonal compartment of neurons wherer it has a role in the rapid elongation of the axon. Hyperphosphorylated forms of Tau are the major components of paired helical filaments
(PHF) found in the neurofibrillary tangles which characterize Alzheimer’s disease. Alternate splicing of tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of tau.

Applications:
Suitable for use in ELISA, Western Blot and Immunhistochemsitry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml detected tau proteins (50-70kD) in rat brain preparations.
Immunohistochemistry: 20ug/ml detected tau proteins in rat brain sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.T.147Affinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human fetal heat-stable MAPS.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes several bands of human Tau protein between 50kD and 70kD. Species Crossreactivity: mouse. Species sequence homology: rat and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kosik KS, et. al., Neuron 1: 817–825, (1988). Kowall NW, et. al., Ann Neurol. 22: 639–643, (1987). McKee AC, et. al., Ann Neurol 26: 652–659, (1989). Kosik KS, et. al., J Neurosci 7: 3142–3153, (1987). Markesbery WR, et. al., Neurobiology of Aging 14: 303–307, (1993). Joachim CL, et. al., J Neuropath Exp Neurol 46: 611–622, (1987)