Technical Data
T1030-01
Tau (Tau Protein, Microtubule-associated Protein, MAP)
Description:
Tau is a type II microtubule associated protein or MAP. Alternative splicing gives rise to 4–5 different forms of the tau protein (55–62kD). Tau is located in the axons and dendrites of nerve cells where it accelerates tubulin polymerization and crosslinks microtubule fibers into stabilized bundles. In this way, the tau protein promotes axon growth. Tau can also induce axonlike projections when expressed at high level in non-neuronal cells. All 6 tau isoforms of the CNS are expressed mainly in axons of the normal brain, but to a lesser extent tau may also be found in astrocytes. A 7th isoform, ‘Big Tau’, is expressed in peripheral nervous system. Tau is altered in Alzheimer’s disease to form paired helical filaments in neurofibrillary tangles, neurofilament threads and senile plaque neurites.

Applications:
Suitable for use in Electron Microscopy, Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Electron Microscopy: 5-25ug/ml
ELISA: 0.1-0.5ug/ml
Western Blot: 0.5-1ug/ml
Immunohistochemistry: 5-25ug/ml; Use in frozen sections, alcohol-fixed paraffin-embedded sections, or Bouin’s-fixed sections. Note: For alcohol-fixed paraffin-embedded section, antigen retrieval not necessary.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13G286Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceBovineMouse
Concentration:
~0.5mg/ml
Immunogen:
Bovine tau.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Recognizes an epitope near the C-terminal region (aa404–441) of human tau. It reacts with all 7 isoforms of human tau (binding-competent and binding-incompetent), A68 (an abnormal phosphorylated derivative of tau), bovine tau and human NeuroFibrillary Tangles (NFTs). Reactivity is independent of the state of tau phosphorylation. Some crossreactivity with MAP2 is also observed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kosik, K.S., et al., Neuron 1: 817-825 (1988). 2. Bramblett, G.T., et al., Lab. Invest. 66: 212-222 (1992). 3. Lee, V.M.-Y., et al., Science 251: 675-678 (1991). 4. Schmidt, M.L., et al., Amer. J. Pathology 136(5): 1069-1075 (1990). 5. Trojanowski, J.Q., et al., J. Histochem. Cytochem. 37: 209-215 (1989). 6. Trojanowski, J.Q., et al., Brain Pathol. 3: 45-54 (1993). 7. Merrick, S.E., et al., J. Biol. Chem. 271(10): 5589-5594 (1996).