Technical Data
T1030-01A
Tau (Tau Protein, Microtubule-associated Protein Tau, MAPT, Neurofibrillary Tangle Protein, Paired Helical Filament-tau, PHF-tau, MAPTL, MTBT1)
Description:
The microtubule-associated proteins (MAPs) coassemble with tubulin into microtubules in vitro. Microtubule-associated protein tau appears to be enriched in axons. Tau are composed of 352 to 441aa. The isoforms differ from each other by the presence or absence of 29-aa or 58-aa inserts located in the N terminus and a 31-amino repeat located in the C terminus. Tau is important in establishing and maintaining neuronal morphology and is a major component of the neurofibrillary tangles (NFTs) characteristic of Alzheimer's brain. Microtubule-associated protein tau (MTBT1) is mapped to chromosome 17q21.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry (paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with 1.2% sodium acetate . Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
Mab10K363Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
As reported
Immunogen:
Bovine microtubule-associated proteins (MAPs).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Specificity:
Recognizes Tau. Species Crossreactivity: human, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.