Technical Data
T1030-04
Tau (Tau Protein, Microtubule-associated Protein, MAP)
Description:
Tau protein is named for its ability to serve as a bridge for microtubule formation and stabilization. Tau in PNS neurons is 758 amino acids (aa) in length and 110 kD in size. It migrates anomalously in SDS-page. Its N-terminus binds plasma membrane components while its C-terminus binds microtubules. The microtubule-binding region contains four consecutive Tau/MAP repeats between aa 561-685. Each repeat is 30 aa long, Ser/Thr rich, and shows a PGGG motif. Multiple isoforms exist, ranging from 316 to 776 aa in length. Various deletions occur within the first 500 aa, with some isoforms also showing an absence of the second Tau repeat (aa 592-622). Between aa 624-756, human Tau protein shows absolute identity to mouse and dog Tau.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml. Detection limit is ~3ng/well.
Western Blot: 1-2ug/ml. Detection limit is ~5ng/lane under reducing and non-reducing conditions
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17H166Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
E. coli-derived recombinant human Tau (aa624-756) Accession # P10636
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human Tau.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.