Technical Data
T2015
T Cell Adaptor Protein (pp36)
Description:
Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.

Application(s):
ELISA: 1:100,000
Western Blotting: 1:1,000
Immunohistochemistry: 1:300

ELISA: Antibody titers and specificity were analyzed by ELISA assay using control samples (varying concentration of GST-EPOr) and control anti GST antibody. Affinity purified antibody was found to detect EPOr at concentrations of 50 ng/ml. Neutralization of ligand binding has been detected using Erytholukemia cell line TF-1 (3000 EPO receptors/cell) to block Iodine-labeled erythropoietin ligand binding. Western blot concentration used 10 ug/ml. It will recognize EPOr-GST at its migration of 49 kD on SDS-PAGE. Good secondary anti-sheep reagents are essential for this assay. Recommended use of rabbit anti-sheep rather than donkey anti-sheep antibodies as a secondary labeling reagents.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide CHRLPGSYDSTSSDSLYPRGI corresponding to #29-48 amino acids at N-terminus of human pp36, a tyrosine-phosporylated adaptor protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide before the addition of 40% glycerol.
Specificity:
Binds to a 36-40kD protein in normal tissues mouse lung and heart. Larger size band approx. 40-45 Kd were seen in human tumor cell lines e.g. T cell line Jurkatt and K562 cells. Crossreacts to mouse p36 and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.