		Technical Data
T2399-14	Telomerase, Human (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

Description:
Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. They regulate several crucial cellular functions and participate in the control of complex phenotypes, such as aging and cancer. Cell immortalization is strongly associated with the stabilization of telomere length. Therefore, it is suggested that cancer cells achieve immortalization in part through illegitimate activation of telomerase expression. hTERT is the rate-limiting catalytic subunit of telomerase.

Applications:
Suitable for use in Flow Cytometry/FACS analysis, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Recomme:nded Secondary Antibodies:
I1905-18D IgM (X-Adsorbed) (FITC) Pab GtxMo
I1905-04A IgM (X-Adsorbed) (PE) Pab GtxMo
I1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo
I1905-15U IgM (AP) Pab GtxMo

Positive Controls:
293T whole cell lysates, HeLa whole cell lysates

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgM	4i236	Ascites

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

Full-length recombinant human TERT from insect cells. Cellular Localization: Nuclear

Purity:

Ascites

Form

Supplied as liquid, 0.05% sodium azide.

Specificity:

Recognizes human Telomerase at ~127kD. May see an additional background band at ~110kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Masutomi, K., et al. PNAS. 102(23): 8222-8227 (2005). 2. Jagadeesh, S., et al. Genistein Represses Telomerase Activity via Both Transcriptional Posttranslational Mechanisms in Human Prostate Cancer Cells. 66: 2107-2115 (2006) 3. Masutomi K et al. Cell. 114(2): 241-53 (2003). 4. Zhang Q, Shi S, Liu Y, et al. J Immunol. 183(12):7787-98 (2009).