Technical Data
T2399-14
Telomerase, Human (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)
Description:
Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. They regulate several crucial cellular functions and participate in the control of complex phenotypes, such as aging and cancer. Cell immortalization is strongly associated with the stabilization of telomere length. Therefore, it is suggested that cancer cells achieve immortalization in part through illegitimate activation of telomerase expression. hTERT is the rate-limiting catalytic subunit of telomerase.

Applications:
Suitable for use in Flow Cytometry/FACS analysis, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Recomme:nded Secondary Antibodies:
I1905-18D IgM (X-Adsorbed) (FITC) Pab GtxMo
I1905-04A IgM (X-Adsorbed) (PE) Pab GtxMo
I1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo
I1905-15U IgM (AP) Pab GtxMo

Positive Controls:
293T whole cell lysates, HeLa whole cell lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM4i236Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Full-length recombinant human TERT from insect cells. Cellular Localization: Nuclear
Purity:
Ascites
Form
Supplied as liquid, 0.05% sodium azide.
Specificity:
Recognizes human Telomerase at ~127kD. May see an additional background band at ~110kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Masutomi, K., et al. PNAS. 102(23): 8222-8227 (2005). 2. Jagadeesh, S., et al. Genistein Represses Telomerase Activity via Both Transcriptional Posttranslational Mechanisms in Human Prostate Cancer Cells. 66: 2107-2115 (2006) 3. Masutomi K et al. Cell. 114(2): 241-53 (2003). 4. Zhang Q, Shi S, Liu Y, et al. J Immunol. 183(12):7787-98 (2009).