Technical Data
T2399-80
Telomerase, Control Peptide (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)
50ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Control Peptide for T2399-85 and T2399-90

A 23aa Peptide sequence mapping near the N-terminus of yeast telomerase/Est2. The 23 AA yeast control peptide has no significant sequence homology with mammalian telomerases.

The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities. Most recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling. (2).

Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2 1122 aa; ~127kD). hEST2 was localized in the nucleus. Yeast telomerase is 988 aa protein.
Source: Yeast
Purity: Highly purified
Concentration: 1mg/ml
Form: Supplied as a liquid in PBS, pH 7.2
Applications: Suitable for use in ELISA or antibody blocking. Other applications not tested.
Recommended Dilutions: ELISA: 5-10ug/1us of affinity pure IgG or 1ul antiserum.
Optimal dilution to be determined by researcher
Specificity: A 23aa Peptide sequence mapping near the N-terminus of yeast telomerase/Est2. The 23 AA yeast control peptide has no significant sequence homology with mammalian telomerases.
Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Nakamura Tm et al (1997) Science 277, 955-959; 2. Meyerson M et al (1997) Cell 90, 785-795; Greenberg RA et al (1998) Oncogene in press; Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.