Technical Data
Tetracycline Hydrochloride
Biochemicals Storage: -20CShipping: RT
Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability.

Applications: Recommended for use in cell culture applications.

Recommended Dilutions:
Cell Culture: 10mg/L. Stable at 37C for 4 days.

Solubility: Water soluble at 5-10g/100ml
Conforms to the following USP28 References:
Appearance: Yellow crystalline powder
Purity: 95-105%
Potency (Assay): 900ug/mg
Identification A (IR): Conforms
Identification B (UV): Conforms
Identification C (HPLC): Conforms
Crystallinity: Conforms
Loss on Drying: 2.0%
Heavy Metals: 0.005%
4-Epianhydrotetracycline: 2%
Anhydrotetracycline: 0.5%
pH (10mg/ml): 1.8-2.8
Specific Optical Rotation: -240-255
Individual Related Substance: 3%
Total Unknown Related Substance: 5%
Bulk Density: 0.42-0.55g/ml
Residual Solvent:
acetone: 0.02%
n-butanol: 0.2%

Tallow derivatives: Produced on the basis of chemical synthetic process; no any animal material contained during the whole production process and also no raw materials are used which are generated from animals during production.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O. Filter sterilize, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. Further dilutions can be made in assay buffer.

CAS Number:

Molecular Formula:

Molecular Weight:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Merck 13: 9271. 2. Beilstein 14(IV) 2627.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.