Technical Data
Media
Tetrathionate Broth Base (Powder)
T3500
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/L:
Casein digest peptone 2.5
Sodium thiosulfate30.0
Peptic digest of animal tissue 2.5
Bile salts 1.0
Calcium Carbonate10.0
Total: 46.0g/L
A standard methods medium for enrichment of Salmonella from samples of water, food and clinical material.

Appearance:
White, homogeneous, free flowing powder

Solubility:
Colorless, clear, complete

pH:
As Reported

Components shown as g/L:

Casein digest peptone 2.5
Sodium thiosulfate 30.0
Peptic digest of animal tissue 2.5
Bile salts 1.0
Calcium carbonate 10.0

Total: 46.0g/L

Microbiological Tests:
Microoranisms:
Escherichia coli ATCC 25922 - Inhibition
Salmonella enteritidis ATCC13076 - Good Recovery
Salmonella typhimurium ATCC 14028 - Good Recovery
Shigella flexneri ATCC 12022 - Inhibition

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions per Liter: Dissolve 46g per liter of ddH2O, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.