Technical Data
Thioredoxin 1
Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase. Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction. A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation. Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1. Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury.

Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:400. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not Determined
Synthetic peptide corresponding to residues surrounding Ala9 of human Thioredoxin 1.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of total human Thioredoxin 1 at ~12kD. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14. 2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404. 3. Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606. 4. Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367. 5. Callister, M.E. et al. (2006) Thorax 61, 521-527.