Technical Data
T5010-10H
Thioredoxin 1
Description:
Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase. Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction. A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation. Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1. Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:400. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG7H76Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Ala9 of human Thioredoxin 1.
Purity:
Supernatant
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human Thioredoxin 1 at ~12kD. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14. 2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404. 3. Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606. 4. Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367. 5. Callister, M.E. et al. (2006) Thorax 61, 521-527.