Technical Data
T5010-14A
Thioredoxin Reductase 1 (TrxR1)
Description:
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 1-2ug/ml of this lot detected thioredoxin reductase 1 in RIPA lysates from rat brain microsomal preparation and HeLa cells.
Immunocytochemistry: Has been reported by an independent laboratory to detect thioredoxin reductase 1 in U937 cells stained with DAPI and CMXRos.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.6mg/ml
Immunogen:
Peptide corresponding to aa137-150 (C-PHRIKATNNKGKEK) of human thioredoxin reductase 1
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes thioredoxin reductase 1, Mr 58kD. An additional protein was also detected, Mr ~70kD, that was inhibited by the immunizing peptide. Species Crossreactivity: Rat and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lee, S.R., et al., J. Biol. Chem. 274: 4722-4734, 1999. 2. Lee, S.R., et al., Proc. Natl. Acad. Sci. USA 97: 2521-2526, 2000.