Technical Data
T5010-14B
Thioredoxin Reductase 1 (Thioredoxin Reductase 1 Cytoplasmic, Thioredoxin Reductase TR1, TR1, TR 1, TR, TRXR 1, TRXR1, TRXR-1, TXNR, TXNRD1, Gene-associated with Retinoid IFN-induced Mortality 12 Protein, GRIM 12, GRIM-12, KM-102-derived Reductase-like Fa
Description:
This protein is a pyridine nucleotide oxidoreductase which reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. It is thought to function as a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity. Alternative splicing results in several transcript variants encoding the same protein. Additional transcript variants have been described but their biological validity has not been determined.

Applications:
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa485-497, TKRSGASILQAGC, representing the C-Terminus of the human protein, according to NP_003321.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Thioredoxin Reductase 1. Species crossreactivity: porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gasdaska PY et al. Cloning and sequencing of a human thioredoxin reductase. FEBS Lett 373:5-9 (1995). PubMed: 7589432