		Technical Data
T5048-04	Thrombomodulin (CD141, TM, Fetomodulin)

Description:
Thrombomodulin is a 110-120kD, endothelial cell-specific type I transmembrane glycoprotein. It binds thrombin, rendering it unavailable for clotting, and activates protein C, which also serves as an anticoagulant. Mouse Thrombomodulin is 577aa in length. It contains a 510aa extracellular domain (ECD) (aa17-517) that shows one C-type lectin domain (aa31-167) and six EGF-like regions (aa240-480). Thrombin binds to aa403-480. One isoform may exist that shows a deletion of the C-terminal 86 amino acids (aa492-577). Over aa17-517, mouse Thrombomodulin is 66%, 85% and 67% aa identical to human, rat and canine TM, respectively.

Applications:
Suitable for use in Flow Cytometry, Direct ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 bEnd.3 cells in a total reaction volume <200ul.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse Thrombomodulin is ~5ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Thrombomodulin is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Immunohistochemistry: 25ug/ml detects Thrombomodulin in frozen sections of normal mouse kidney and heart.
Immunocytochemistry: 10ug/ml. Used with bEnd.3 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Between each step, cells were washed with PBS, BSA.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	8H203	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rat

Concentration:

~0.5mg/ml (after reconstitution)

Immunogen:

Recombinant protein corresponding to aa17-517 from mouse Thrombomodulin expressed in NSO cells (P15306).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Specificity:

Recognizes recombinant mouse Thrombomodulin. Does not crossreact with recombinant human Thrombomodulin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.