Technical Data
T5100-06B
Thrombospondin (TSP)
Description:
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

MW of Antigen: ~450kD (non-reduced)

Epitope: N-terminal half of the central stalk-like region of TSP1 which is recovered as a 50kD fragment after chymotryptic digestion of TSP.1

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, and Blocking studies. Not suitable for Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2hrs at RT (non-reduced only)
Blocking: Order Ab without azide
Optimal dilution to be determined by the researcher.

Positive Control: HUVEC cells.

Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM3F351Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Calcium-replete, native puri ed human TSP from the supernatant of thrombin-activated platelets
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5100-06BX
Specificity:
Blocks anti-angiogenic activity of TSP-1 by inhibiting its binding to TSP-Receptor / CD36. It shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. It inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells and rat smooth muscle cells to TSP.1 It does not inhibit aggregation of thrombin-induced platelets. Species Crossreactivity: human, mouse, rat, bovine, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Prater CA, et. al. J Cell Biol, 1991, 112(5):103140. 1. DeFreitas MF; et al., Neuron, 1995 Aug, 15(2):33343. 2. RayChaudhury A; Journal of Cell Science, 1994 Jan, 107 ( Pt 1):3946. 3. Tolsma SS; Journal of Cell Biology, 1993 Jul, 122(2):497511. 4. Frazier WA. Thrombospondins. Current Opinion in Cell Biology, 1991 Oct, 3(5):7929.