Technical Data
Thrombospondin (TSP) (BSA & Azide Free)
Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Suitable for use in Electron Microscopy, Flow Cytometry. Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation (Native verified): 2ug/mg protein lysate, Use Protein G.
Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilution determined by the researcher.

Positive Control:
Platelets in placenta, megakaryocytes in bone marrow

Cellular Localization:
Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG13F353Affinity Purified
100ul-20CBlue IceHumanMouse
Reduced and alkylated puri ed human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T5100-06D.
Recognizes human Thrombospondin. Shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild crossreaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i.e. at low concentration of Ca2+. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit VM, et al. J Bio Chem, 1986, 261(4):19628. 2. Galvin NJ, et al. J Cell Bio, 1987, 104(5):141322. 3. Dixit VM, et al. Biochemistry, 1985, 24(16):42705