Technical Data
T5100-06P
THBS1 (Thrombospondin-1, TSP1, TSP) (Biotin)
Description:
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-0.4ug/ml.
Western Blot: 0.1-0.2ug/ml. The detection limit for rhThrombospondin-1 is approximately 50 ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
Not determined
Immunogen:
Purified, NS0-derived, recombinant human Thrombospondin-1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Specificity:
Recognizes human Thrombospondin-1. Crossreactivity: Thrombospondin 2 and Thrombospondin 4 (less than 5%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.