Technical Data
Thrombospondin (THBS, TSP, Thrombospondin 1, THBS1, THBS-1, Tsp1, Tsp-1)
Thrombospondin-1 is a 450kD trimeric glycoprotein first described in 1971. The molecule is stored in high concentrations in the alpha granules of platelets, constitutes 25% of platelet-secreted protein, and participates in platelet aggregation and clot formation. It is one of a class of adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. Numerous biological activities have been ascribed to TSP-1, including effects on cell adhesion, migration, proliferation, and angiogenesis. It also plays both complex and controversial role in tumor progression.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceHumanMouse
Not determined
Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum.
Supplied as a liquid, 0.05% sodium azide.
Recognizes human Thrombospondin. Species Crossreactivity: bovine, canine, equine, mouse, porcine, rat, sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit et al. Biochemistry 24 (16): 4270, 1985. 2. Dixit et al. J Bio Chem 261 (4): 1962, 1986. 3. Galvin et al. J Cell Bio 104 (5): 1413, 1987. 4. Irene et al. Experimental Biology and Medicine 226: 726, 2001.