Technical Data
T5100-06Q
Thrombospondin (THBS, TSP, Thrombospondin 1, THBS1, THBS-1, Tsp1, Tsp-1)
Description:
Thrombospondin-1 is a 450kD trimeric glycoprotein first described in 1971. The molecule is stored in high concentrations in the alpha granules of platelets, constitutes 25% of platelet-secreted protein, and participates in platelet aggregation and clot formation. It is one of a class of adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. Numerous biological activities have been ascribed to TSP-1, including effects on cell adhesion, migration, proliferation, and angiogenesis. It also plays both complex and controversial role in tumor progression.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111E355Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum.
Purity:
Purified
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes human Thrombospondin. Species Crossreactivity: bovine, canine, equine, mouse, porcine, rat, sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit et al. Biochemistry 24 (16): 4270, 1985. 2. Dixit et al. J Bio Chem 261 (4): 1962, 1986. 3. Galvin et al. J Cell Bio 104 (5): 1413, 1987. 4. Irene et al. Experimental Biology and Medicine 226: 726, 2001.