Technical Data
T5100-39
Thrombospondin 2 (Thrombospondin-2, THBS2, THBS-2, TSP2, TSP-2)
Description:
Thrombospondin-2 is a disulfide-linked homotrimer that belongs to the small thrombospondin family of secreted modular glycoproteins. It is a matricellular protein that can interact with cell-surface receptors, cytokines and proteases to modulate cell function.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 2-8ug/ml. Can be used as capture antibody in sandwich ELISA with polyclonal detection antibody.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Thrombospondin-2 (aa19-1172).

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2A9L855Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa19-1172 from human Thrombospondin-2 expressed in NS0 cells (P35442).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes human Thrombospondin-2. Does not crossreact with recombinant human Thrombospondin-1, -4, recombinant human MMP-2 or recombinant human CD36.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.