Technical Data
T5470-03
Timeless (Tim1)
Description:
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. The genes period (dPer) and timeless (tim) in Drosophila and frequency (frq) in Neurospora have been proposed to be responsible for their circadian rhythm. In mammals, the suprachiasmatic nuclei (SCN) of the anterior hypothalamus serves as a master circadian clock with a measurable circadian rhythm. In Drosophila, the RNA and protein products of per and tim oscillate and may be involved in rhythm regulation. The levels of per and tim is positively regulated by two basic helix-loop-helix (bHLH)/PAS proteins, dCLOCK and dBMAL1, which heterodimerize and bind to E box enhancers. Translated per and tim are phosphorylated, form heterodimers, and then translocate to the nucleus. Once in the nucleus, PER and TIM function as negative regulators of their own transcription. Recently drosophila homologs of mammalian TIM have been cloned and characterized (1). Human Timeless 1 (hTIM1; 1208aa, chromosome 12q12-13) and mouse TIM1 (1197aa, chromosome 10D3) share 83% identity. Tim1 is weakly expressed in SCN, and in several peripheral tissues (eyes, spleen and testis). Unlike Drosophila, mouse Tim1 does not oscillate in the SCN and is not modulated by light exposure. Human TIM1 and mPER1 specifically inhibit CLOCK-BMAL1-induced transactivation of mPER1 promoter.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. See T5470-02.
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
A mixture of 14aa peptide sequence near the N-terminus of mouse Tim1 and 14aa peptide sequence from the C-terminus of human Tim1 (KLH coupled).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse and human (Tim1). Species Sequence Homology (Mouse Tim1 peptide): Rat (100%), Drosophila (85%), Human (85%). Species Sequence Homology (Human Tim1 peptide): Mouse (57%). Expected to crossreact with rat and Drosophila based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sangoram, A.M., et al., Neuron 21: 1101-1113 (1998). 2. Zylka, M.J., et al., Neuron 21: 1115-1122 (1998). 3. Koike, N., et al., FEBS Lett. 441: 427-431 (1998).