Technical Data
Tie2 (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Mice lacking TIE 1 or TIE 2 are lethal. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis. TIEs appear to be acting downstream of the VEGFRs. Tie-2 (human 1124 AA; mouse 1122 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells and their progenitors, angioblasts. It is also found in placenta and lung, with lower levels in umbilical vein endothelial cells, brain and kidney. Tie-2 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2-Ig-like C2-type, and 3-EGF-like domains.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000
Western Blot: 1-10ug/ml (ECL)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to 18aa, near the N-terminal, extracellular of human Tie2, conjugated to KLH. Species Sequence Homology: mouse and rat; 100%, bovine; 94%.
Purified by immunoaffinity chromatography
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA
Recognizes human Tie2. No significant sequence homology is seem with Tie1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ziegler SF et al (1993) Oncogene 8, 663. 2. Vikkula M et al (1996) Cell 87, 1181. 3. Sato TN et al (1993) PNAS 90, 12056. 4. Dumont DJ et al (1993) Oncogene 8, 1293. 5. Horita K et al (1992) BBRC 189, 1747. 6. Runtig AS et al (1993) Growth factors 9, 99. 7. Dumont DJ et al (1992) Oncogene 8, 1471