Technical Data
T5498-71J
Tie2 (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)
Description:
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Mice lacking TIE 1 or TIE 2 are lethal. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis. TIEs appear to be acting downstream of the VEGFRs. Tie-2 (human 1124 AA; mouse 1122 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells and their progenitors, angioblasts. It is also found in placenta and lung, with lower levels in umbilical vein endothelial cells, brain and kidney. Tie-2 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2-Ig-like C2-type, and 3-EGF-like domains.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000; using 50-100ng of cT5498-71F control peptide/well.
Western Blot: 1-10ug/ml using ECL technique. Native Tie-2 is ~140kD. T5498-71G Tie2, Recombinant, Human, Western Blot Control migrates as 125-135kD due to glycosylation.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 18aa, ~N-terminal, extracellular of human Tie2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.
Specificity:
Recognizes human Tie2. No significant sequence homology is seem with Tie1. Species Sequence Homology: mouse, rat - 100%; bovine - 94%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Ziegler SF et al (1993) Oncogene 8, 663; Vikkula M et al (1996) Cell 87, 1181; Sato TN et al (1993) PNAS 90, 12056; Dumont DJ et al (1993) Oncogene 8, 1293; Horita K et al (1992) BBRC 189, 1747; Runtig AS et al (1993) Growth factors 9, 99; Dumont DJ et al (1992) Oncogene 8, 1471