Technical Data
T5498-72
Tie2 (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)
Description:
TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation: Reported to immunoprecipitate Tie2/TEK.
Western Blot: 0.2-1ug/ml. HUVEC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T5498-72. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections. Not suitable for use in paraf n- embedded sections.
Optimal dilutions to be determined by the researcher.

Positive Control:
HUVEC cell lysate

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k4i64Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Fusion protein corresponding to aa1-745 of human Tie2/TEK with a C-terminal His6-tag expressed in infected SF-9 cells (NM_000459).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes human Tie2/TEK, Mr 145kD. Species Crossreactivity: mouse, rat and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lin, P., et al., Proc. Natl. Acad. Sci. USA 95: 88298834 (1998). 2. Wong, A.L., et al., Circ. Res. 81: 567574 (1997). 3. Peters, K.G., et al., Br. J. Cancer 77: 5156 (1998).