Technical Data
TIS7 (TPA-induced Sequence 7, TIS7 Protein, Interferon-related Developmental Regulator 1, IFRD1, Nerve Growth Factor-inducible Protein PC4, PC4)
Tis7, also known as IFRD1, is a 442aa containing membrane associated, non-nuclear intracellular protein belonging to the IFRD family. A regulatory protein expressed in a variety of tissues, Tis7 is known to play an important role in the regulation of gene activity in the proliferative and/or differentiative pathways induced by NGF. Reports suggest that Tis7 may be an autocrine factor that attenuates or amplifies the initial ligand induced signal and might also augment the adaptive response by stimulating the production of differentiated enterocytes. Tis7 acts as a negative regulator of transcriptional activity and represses expression of genes involved in myogenesis, muscle maintenance, and regeneration in a histone deacetylase dependent manner.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-4ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa70-120 of human Tis7.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human Tis7. Species Crossreactivity: chimpanzee, bovine, canine, macaque, pocine, rhesus monkey. Species sequence homology: mouse, rat, equine, xenopus, chicken, opossum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Varnum, BC. et al. Oncogene. 4:1263-1265 (1989). 2. Vietor, I. et al. J Biol Chem. 280:39795-39801 (2005). 3. Vietor, I. et al. Differentiation. 75:891-897 (2007).