Technical Data
T5580-15
Tissue Inhibitor of Metalloproteinases 1 (Erythroid Potentiating Activity, EPA, Collagenase Inhibitor, CI)
Description:
TIMP-1and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 5-10ug/ml. Recognizes a band at ~28-30kD in reduced samples.
Immunohistochemistry: 1ug/section
ELISA: 5ug/ml
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Full length purified human fibroblast TIMP (Tissue Inhibitor of Metalloproteinase).
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human TIMP (Tissue Inhibitor of Metalloproteinase). Recognizes native and denatured TIMP and TIMP in and out of enzyme/inhibitor complex. Does not crossreact with other human proteins tested. Not known to inhibit biological activity. Species Crossreactivity: rabbit, sheep, bovine, rat, equine and ferret
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.