Technical Data
Tissue Inhibitor of Metalloproteinases 2 (TIMP2) (BSA & Azide Free)
TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP2 may be used to evaluate the invasive nature of some tumor types and determine the tissue disatribution of TIMPs in other tumors such as adenomas and also in normal tissue.

Suitable for use in Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:200 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Conditioned, serum-free medium from (TPA-treated) HFL-1 cells. Pancreatic or colon carcinoma.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a,k3F373Affinity Purified
100ul-20CBlue IceHumanMouse
Synthetic peptide from the N-terminal region of human TIMP2 protein. Cellular Localization: Cytoplasmic
Purified by Protein A affinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See T5585-10A
Recognizes human Tissue Inhibitor of Metalloproteinases 2 at ~21kD. Species Crossreactivity: mouse, rat, rabbit, bovine and guinea pig
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Murray g I, et al. (1998) Gut,43:791797.