Technical Data
T8050-01K
TLR1 (Toll Like Receptor 1, Toll-like Receptor 1 Precursor, CD281, CD281 Antigen, DKFZp547I0610, DKFZp564I0682, KIAA0012, MGC104956, MGC126311, MGC126312, rsc786, Similar to Drosophila Toll Protein, TIL, Toll (Drosophila) Homolog, Toll/Interleukin 1 Recep
Description:
CD281 is known as toll like receptor 1 (TLR1). TLRs recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and serve as an important link between the innate and adaptive immune response through the production of cytokines. TLR1 is co-expressed with TLR2 on monocytes and dendritic cells where they form heterodimers that can recognize peptidoglycan and bacterial lipopeptides, and in particular triacylated lipoproteins.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 10ug/ml
Western Blot: 1-4ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse speen tissue

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
16aa peptide located near the N-terminal of human CD281.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human TLR1 at ~90kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hajjar, A.M. et al. (2001) Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin. J. Immunol. 166: 1519. 2. Janssens, S. and Beyaert, R. (2003) Role of Toll-like receptors in pathogen recognition. Clin. Microbiol. Rev. 16: 637 646.
3. Spitzer, J.H. et al. (2002) Toll-like receptor 1 inhibits Toll-like receptor 4 signaling in endothelial cells. Eur. J. Immunol. 32: 11821187.