Technical Data
T8050-03
TLR1 (Toll Like Receptor 1)
Description:
The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Flow Cytometry: 2-4ug/1x10e6 cells
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Ramos cell lysate (87kD)

Storage and Stability:
May be stored at 4░C for short-term only. For long-term storage, store at -20░C. Aliquots are stable for at least 12 months at -20░C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20░CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a sequence within aa400-450 of human TRL1 (KLH coupled).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human TRL1. Does not cross-react with mouse TLR1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wyllie, D.H., et al. J Immunol. 165: 7125 (2000). 2. Visintin, A., et al. J. Immunol. 166: 249 (2001). 3. Liu S, et al. Infect Immun 70(7):3433-42 (2002) 4. Takeuchi O, et al. J Immunolá 169(1):10-14 (2002)