Technical Data
T8065-11A
Topoisomerase IIa
Description:
DNA topoisomerase I and II are nuclear enzymes, and type II consist of two highly homologous isoforms: topoisomerase IIa and IIb. These enzymes regulate the topology of DNA, maintain genomic integrity and are essential for processes such as DNA replication, recombination, transcription and chromosome segregation by allowing DNA strands to pass through each other (1). Topoisomerase I nicks and rejoins one strand of the duplex DNA, but topoisomerase II transiently breaks and closes double-stranded DNA (2). Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g. etoposide) also convert topoisomerases to nucleases, and the enzyme is usually trapped as an intermediate, covalently bound to the 5' end of the cleaved DNA strand(s). This intermediate leads again to genomic instability and cell death, and thus these agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs (3). Ca2+ regulated phosphorylation of topoisomerase IIa Ser1106 modulates the activity of this enzyme as well as its sensitivity to targeting drugs (4).

Applications:
Suitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (KLH-coupled) derived from the sequence of human Topoisomerase IIa.
Purity:
Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol.
Specificity:
Detects endogenous levels of total Topoisomerase IIa protein. Species Crossreactivity: Human Mouse Rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Wang, J.C. (2002) Nat. Rev. Mol. Cell. Biol. 3, 430440. (2) Pulleyblank, .E. (1997) Science 277, 648649. (3) Li, T.K. and Liu, L.F. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 5377. (4) Chikamori, K. et al. (2003) J. Biol. Chem. 278, 1269612702.