Technical Data
Topoisomerase IIa
DNA topoisomerase I and II are nuclear enzymes, and type II consist of two highly homologous isoforms: topoisomerase IIa and IIb. These enzymes regulate the topology of DNA, maintain genomic integrity and are essential for processes such as DNA replication, recombination, transcription and chromosome segregation by allowing DNA strands to pass through each other. Topoisomerase I nicks and rejoins one strand of the duplex DNA, but topoisomerase II transiently breaks and closes double-stranded DNA. Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g. etoposide) also convert topoisomerases to nucleases, and the enzyme is usually trapped as an intermediate, covalently bound to the 5' end of the cleaved DNA strand(s). This intermediate leads again to genomic instability and cell death, and thus these agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs. Ca2+ regulated phosphorylation of topoisomerase IIa Ser1106 modulates the activity of this enzyme as well as its sensitivity to targeting drugs.

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to the sequence of human Topoisomerase IIa.
Protein A and peptide affinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Recognizes endogenous levels of total human Topoisomerase IIa protein, Mr 190kD. Species Crossreactivity: Mouse, rat, monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Wang, J.C. (2002) Nat. Rev. Mol. Cell. Biol. 3, 430440. (2) Pulleyblank, .E. (1997) Science 277, 648649. (3) Li, T.K. and Liu, L.F. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 5377. (4) Chikamori, K. et al. (2003) J. Biol. Chem. 278, 1269612702.