Technical Data
T8065-12J
Topoisomerase II alpha (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2A, TOP2)
Description:
Topo II-a plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo II-a is also implicated in drug resistance of tumor cells.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 5-10ug/ml for 2h at RT
Immunoprecipitation: 10ug/mg protein lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
Raji cells or exponentially growing any cultured human cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to aa1513-1530 from the C-terminus of human topoisomerase II-a.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Topoisomerase II alpha.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.