Technical Data
T8065-14
Topoisomerase II (DNA) alpha
Description:
Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin):1:20 for 20 min at RT. Antigen retrieval of boiling in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at RT for 20 min is required. Optimal dilutions to be determined by researcher.

Positive Control: Tonsil

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k5G228Supernatant
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Lysate of the human histiocytic leukemia cell line U937.
Purity:
Culture supernatant.
Form
Supplied as a liquid in PBS, 0.09% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes Topoisomerase II alpha. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kreipe H, et al. (1993) Am J Pathol, 142:3-9.
2. Rudolph P, et al. (1995) Am J Pathol, 147:1615.
3. Kreipe H, et al. (1993) Am J Pathol, 142:651-657.
4. Claviez A, et al. (1994) Ann Hematol, 68:61-66.
5. Woessner r D, et al. (1991) Cell Growth Differ, 2:209-214.
6. Boege F, et al. (1995) Am J Pathol, 146:1302-1308.