Technical Data
T8076-35B
Toxoplasma gondii (Major Surface Antigen p30)
Description:
Toxoplasma gondii is a protozoan parasite that can infect healthy individuals and also cause congenital defects in the fetus and severe infection in immunocom- promised hosts. This antibody will help detect most strains of Toxoplasma gondii as it recognizes the highly conserved and prominent P30 surface antigen.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:10-1:20 for 1 hour at RT. Requires antigen retrieval.
Optimal dilutions to be determined by the researcher.

Positive Controls:
Toxoplasma gondii-infected cerebellum. Cytoplasm of infected cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2aTP3Affinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not Determined
Immunogen:
Tachyzoites lysate
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 1% BSA, 15mM sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes Toxoplasma gondii P30 Antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Decoster A, Gontier P, Dehecq E, et al.. Detection of anti-Toxoplasma immunoglobulin A antibodies by Platelia-Toxo IgA directed against P30 and by IMx Toxo IgA for diagnosis of acquired and congenital toxoplasmosis. Journal of Clinical Microbiology. 33(8):2206-2208 (1995). 2.Bonhomme A, Thirion C, Boulanger F, et al.. Toxoplasma gondii-structure variations of the antigen P30. Parasitology. 108:281-287 (1994). 3.Godard I, Estaquier J, Zenner L, et al.. Antigenicity and immunogenicity of P30-derived peptides in experimental models of toxoplasmosis. Molecular Immunology. 31(17):1353-1363 (1994). 4.Kim K, B low R, Kampmeier J et al.. Conformationally appropriate expression of the Toxoplasma antigen SAG1 (p30) in CHO cells. Infection and Immunity. American Society for Microbiology. 62(1):203-209 (1994). 5. Mineo J R, McLeod R, Mack D et al.. Antibodies to Toxoplasma gondii major surface protein (SAG-1, P30) inhibit infection of host cells and are pro- duced in murine intestine after peroral infection. The Journal of Immunology. 150(9):3951-3964 (1993).