Technical Data
TRAF2 Binding Protein (TRAF2-binding Protein, T2BP, Putative MAPK-activating Protein PM14, Putative NF-kappa-B-activating Protein 20, TRAF-interacting Protein with Forkhead-associated Domain, TRAF-interacting Protein with FHA Domain-containing Protein A,
T2BP, a novel 184aa protein, is human homolog of mouse Tifa. It consists of a central phosphopeptide-binding FHA domain and a C-terminal TRAF6-binding motif. T2BP mediates multiple protein-protein interactions and plays a significant role in innate immunity, adaptive immunity and inflammation. It interacts with TRAF2 and is involved in TNF-mediated signaling. It induces oligomerization and polyubiquitination of TRAF6 leading to activation of TAK1 and IKK in a proteasome-independent mechanism and mediates NF-kappaB and JNK activation. It enhances IRAK1/TRAF6 interactions and mediates IL-1 signaling suggesting a novel regulation of innate immunity and inflammation.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa150-162 of human T2BP, TIFA.
Supplied as a liquid.
Recognizes human TRAF2 Binding Protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kanamori Biochem Biophys Res Commun.,290(3):1108-13 (2002 Jan 25) 2. Hiroshi J. Biol. Chem., 278 (14); 12144-12150 (April 4, 2003) 3. Ea PNAS 101 (43); 15318-15323 (October 26, 2004).