Technical Data
TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Human soluble Trail also called TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand AND TL2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids and having a molecular mass of 19.6kD. TRAIL is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Arg-Glu-Arg-Gly-Pro.

Biological Activity:
Recombinant Human TRAIL is fully biologically active when compared to standard. The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensetive U343MG cells, ED50 for this effect is 1-3 ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 97% (RP-HPLC, SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder from 150mM sodium chloride, 50mM sodium phosphate, pH 7. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to >0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Human Brain Astrocytes Mediate TRAIL-mediated Apoptosis after Treatment with IFN-gamma. Yonsei Med J 2006 Jun 30;47(3):354-8. 2. [Influence of Chemotherapy on Expression of TRAIL in Primary Acute Leukemic Cells.] Zhongguo Shi Yan Xue Ye Xue Za Zhi 2006 Jun;14(3):481-4 3. Improvement of Expression Level and Bioactivity of Soluble Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Apo2L/TRAIL) by a Novel Zinc ion Feeding Strategy. Biotechnol Lett 2006 Jun 24; 4. The antitumor activity of TRAIL and IL-24 with replicating oncolytic adenovirus in colorectal cancer. Cancer Gene Ther 2006 Jun 23; 5. TRAIL-induced apoptosis proceeding from caspase-3-dependent and -independent pathways in distinct HeLa cells. Biochem Biophys Res Commun 2006 Jun 12; 6. [Effects of hTERT RNAi on apoptosis of hepatocellular carcinoma cells induced by TRAIL.] Zhonghua Gan Zang Bing Za Zhi 2006 Jun;14(6):435-40.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.