Technical Data
T8199-01B
Transferrin
Description:
Transferrin, also known as serotransferrin and siderophilin, is an 80kD secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19aa signal sequence and a 679aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin has sequence homology of 73% to mouse and rat Transferrin.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Transferrin under non-reducing conditions only. Lysates were prepared from human liver tissue. PVDF membrane was probed with 2ug/ml of T8199-01B followed by HRP­conjugated Anti­Mouse IgG secondary antibody. A specific band was detected for Transferrin at ~90kD. Use of this antibody under reducing conditions is not recommended.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-75E: IgG1 (HRP) Pab Rb x Mo
I1904-79M: IgG1 (HRP) Pab Gt x Mo

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H186Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant corresponding to aa20-698 of human Transferrin expressed in SF 21 insect cells (AAH59367).
Purity:
Purified by Protein G immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human Transferrin in direct ELISAs and Western Blots.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.