Technical Data
Transferrin, also known as serotransferrin and siderophilin, is an 80kD secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19aa signal sequence and a 679aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin has sequence homology of 73% to mouse and rat Transferrin.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml using human liver tissue
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG19H186Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml (after reconstitution)
Recombinant protein corresponding to aa20-698 from human Transferrin expressed in SF 21 insect cells (AAH59367).
Purified by Protein G affinity chromatography
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human Transferrin in direct ELISAs and Western Blots.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.