Technical Data
T8199-03
Transferrin (Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin, DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin)
Description:
Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1,8002,700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Functional Assays. Other applications not tested.

Recommended Dilution:
ELISA: 1:500- 1:5000.
Immunohistochemistry (paraffin): 10ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections using sodium citrate buffer, pH 6.0.
Functional Assays: Removal of sodium azide is recommended.
Optimal dilutions to be determined by the researcher.

Positive Control Tissue:
Placental villi

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab)2 (HRP) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110B2492Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human transferrin
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes N-terminal domain of human transferrin. Inhibits the porliferation of Con-A stimulated blood lymphocytes in functional assays. Does not react with mouse, rabbit and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.