Technical Data
T8199-03
Transferrin (Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin, DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin)
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Technical Data |
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T8199-03 |
Transferrin (Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin, DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin) |
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Description: Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1,800–2,700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Applications: Suitable for use in ELISA, Immunohistochemistry and Functional Assays. Other applications not tested. Recommended Dilution: ELISA: 1:500- 1:5000. Immunohistochemistry (paraffin): 10ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections using sodium citrate buffer, pH 6.0. Functional Assays: Removal of sodium azide is recommended. Optimal dilutions to be determined by the researcher. Positive Control Tissue: Placental villi Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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