Technical Data
T8199-04R
Transferrin (Tf) (HRP)
Description:
Transferrin (Tf, human chromosome 3, 679aa), a serum glycoprotein of ~80kD and synthesized in the liver, is the primary protein of inter-organ transport of nonheme iron. Tf can bind two iron atoms. Tf binds to membrane Transferrin receptors (TfRs) and taken up by endocytosis. Iron is released from Tf, within acidic endosomes, into the cytoplasm apparently through the action of DMT1. The apoTf-TfR complex is returned to the cell surface, where, apo-Tf dissociates from TfR at the extracellular pH. The classical TfR, now termed TfR1, is a homodimeric (95kD subunits) type II membrane glycoprotein that binds two molecules of Tf. Human TfR1 (human 760aa; mouse 763aa) has a cytoplasmic domain 1-67aa, 68-88aa TM, and 89-760aa as extracellular domains. A monomeric serum form or soluble TfR1 (~80kD) also exists that lacks residues 1-100 aa. Recently, a second Tf receptor, TfR2, has been cloned and characterized. TfR2 shares 45% identity with TfR1, and 27% with PMSA. Several variants of Tf have been identified with varying iron binding ability.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000-1:10,000
Western Blot:1:1000-1:5000 using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseGoat
Concentration:
Not Determined
Immunogen:
Purified mouse serum Transferrin (Tf)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in stabilizing buffer, 0.1% BSA, 0.1% ProClin-300.
Specificity:
Recognizes mouse Transferrin (Tf) at ~80kD. Reacts poorly with human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bowman, B. H. et al (1988) Adv. Genet. 25: 1-38. 2. Evans, R. W. et al (1982) Biochem. J. 201: 19-26. 3. MacGillivray, R. T. A et al (1982) PNAS 79: 2504-2508. 4. Park, I. et al (1985) PNAS 82, 3149. 5. Uzan, G. et al (1984) BBRC 119, 273. 6. Yang, F. et al (1984) PNAS 81, 2752-2756. 7. Nelson N et al (1999) EMBO J. 18, 4361. 8. Cairo G et al (2000) Biochem. J. 352, 241-250